Use of a fish waste-derived biofertilizer in the biomass production of Chlorella vulgaris
DOI:
https://doi.org/10.70577/cieninter.v4i3.135Keywords:
Chlorella vulgaris, optimized fish biol, cell density, total chlorophyll, dry biomass.Abstract
This study evaluated how a fish waste-based biofertilizer supplemented with three N:P ratios influences the biomass production of Chlorella vulgaris. An increasing stimulus experimental design was set up with a control group (Guillar f) and three treatments (biofertilizer: 10:1, 15:1, and 20:1 N:P), using three replicates per condition for a total of 12 experimental units arranged in a completely Randomized Design (CRD). Daily cell growth was tracked via Neubauer chamber counts and supported by optical density readings at 680 nm (OD680). Population density was positively shaped by the enriched biol, with the 20.1 N:P ratio (T3) yielding the best results on Day 11, reaching 10,78 × 106 cells · mL-1. By end of the trial, the 20:1 N:P treatment outperformed both the other formulations and the control group in dry biomass gravimetric yield (0,6560 ± 0.0461 g · L-1) and total chlorophyll (10,9274 mg · L-1). Ultimately, this optimized fish biol serves as an efficient biotechnological pathway for microalgal biomass production.
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